Identification, Quantification and Kinetic Study of Carotenoids and Lipids in Rhodotorula toruloides CBS 14 Cultivated on Wheat Straw Hydrolysate

Production of carotenoids and lipids by Rhodotorula toruloides CBS 14 cultivated on wheat straw hydrolysate was investigated. An ultra-high-performance liquid chromatography (UHPLC) method for carotenoid quantification developed validated. Saponification effects individual were identified, lipid kinetics during cultivation determined. The β-carotene, γ-carotene, torularhodin, torulene identified; β-carotene the major carotenoid, reaching a maximum 1.48 mg/100 g dry weight. Recoveries between 66% 76%, except which had lower recoveries due to saponification effects. Total content in saponified unsaponified yeast extract, respectively, determined UHPLC or photometer, 1.99 4.02 mg β-EQ/100 Growth showed positive correlation accumulation. at all time points. At end cultivation, triacylglycerols (TAGs) class, with 58.1% ± 3.32% total lipids. There also high proportion free fatty acids, from 20.5% 41.8% Oleic acid (C18:1) acid. yield 0.13 g/g sugar consumed.